3,5-diiodo-L-thyronine affects structural and metabolic features of skeletal muscle mitochondria in high-fat-diet fed rats producing a co-adaptation to the glycolytic fiber phenotype

Elena Silvestri, Federica Cioffi, Rita De Matteis, Rosalba Senese, Pieter de Lange, Maria Coppola, Anna M. Salzano, Andrea Scaloni, Michele Ceccarelli, Fernando Goglia, Antonia Lanni, Maria Moreno, Assunta Lombardi

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Abstract

Hyperlipidemic state-associated perturbations in the network of factors controlling mitochondrial functions, i. e., morphogenesis machinery and metabolic sensor proteins, produce metabolic inflexibility, insulin resistance and reduced oxidative capacity in skeletal muscle. Moreover, intramyocellular lipid (IMCL) accumulation leads to tissue damage and inflammation. The administration of the naturally occurring metabolite 3,5-diiodo-L-thyronine (T2) with thyromimetic actions to high fat diet (HFD)-fed rats exerts a systemic hypolipidemic effect, which produces a lack of IMCL accumulation, a shift toward glycolytic fibers and amelioration of insulin sensitivity in gastrocnemius muscle. In this study, an integrated approach combining large-scale expression profile and functional analyses was used to characterize the response of skeletal muscle mitochondria to T2 during a HFD regimen. Long-term T2 administration to HDF rats induced a glycolytic phenotype of gastrocnemius muscle as well as an adaptation of mitochondria to the fiber type, with a decreased representation of enzymes involved in mitochondrial oxidative metabolism. At the same time, T2 stimulated the activity of individual respiratory complex I, IV, and V. Moreover, T2 prevented the HFD-associated increase in the expression of peroxisome proliferative activated receptor γ coactivator-17alpha; and dynamin-1-like protein as well as mitochondrial morphological aberrations, favoring the appearance of tubular and tethered organelles in the intermyofibrillar regions. Remarkably, T2 reverted the HDF-associated expression pattern of proinflammatory factors, such as p65 subunit of NF-kB, and increased the fiber-specific immunoreactivity of adipose differentiation-related protein in lipid droplets. All together, these results further support a role of T2 in counteracting in vivo some of the HFD-induced impairment in structural/metabolic features of skeletal muscle by impacting the mitochondrial phenotype.

LanguageEnglish
Article number194
JournalFrontiers in Psychiatry
Volume9
Issue numberMAR
DOIs
Publication statusPublished - 9 Mar 2018

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Muscle Mitochondrion
High Fat Diet
Skeletal Muscle
Phenotype
Insulin Resistance
Dynamin I
Electron Transport Complex I
Lipids
Peroxisomes
NF-kappa B
Morphogenesis
Organelles
Mitochondria
Proteins
3,5-diiodothyronine
Inflammation
Enzymes

Keywords

  • Adipose differentiation-related protein
  • Diiodothyronine
  • Inflammation
  • Mitochondrial dynamics
  • Mitochondrion
  • Proteomics
  • Reactive oxygen species
  • Skeletal muscle

ASJC Scopus subject areas

  • Psychiatry and Mental health

Cite this

3,5-diiodo-L-thyronine affects structural and metabolic features of skeletal muscle mitochondria in high-fat-diet fed rats producing a co-adaptation to the glycolytic fiber phenotype. / Silvestri, Elena; Cioffi, Federica; De Matteis, Rita; Senese, Rosalba; de Lange, Pieter; Coppola, Maria; Salzano, Anna M.; Scaloni, Andrea; Ceccarelli, Michele; Goglia, Fernando; Lanni, Antonia; Moreno, Maria; Lombardi, Assunta.

In: Frontiers in Psychiatry, Vol. 9, No. MAR, 194, 09.03.2018.

Research output: Contribution to journalArticle

Silvestri, E, Cioffi, F, De Matteis, R, Senese, R, de Lange, P, Coppola, M, Salzano, AM, Scaloni, A, Ceccarelli, M, Goglia, F, Lanni, A, Moreno, M & Lombardi, A 2018, '3,5-diiodo-L-thyronine affects structural and metabolic features of skeletal muscle mitochondria in high-fat-diet fed rats producing a co-adaptation to the glycolytic fiber phenotype' Frontiers in Psychiatry, vol. 9, no. MAR, 194. https://doi.org/10.3389/fphys.2018.00194
Silvestri, Elena ; Cioffi, Federica ; De Matteis, Rita ; Senese, Rosalba ; de Lange, Pieter ; Coppola, Maria ; Salzano, Anna M. ; Scaloni, Andrea ; Ceccarelli, Michele ; Goglia, Fernando ; Lanni, Antonia ; Moreno, Maria ; Lombardi, Assunta. / 3,5-diiodo-L-thyronine affects structural and metabolic features of skeletal muscle mitochondria in high-fat-diet fed rats producing a co-adaptation to the glycolytic fiber phenotype. In: Frontiers in Psychiatry. 2018 ; Vol. 9, No. MAR.
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abstract = "Hyperlipidemic state-associated perturbations in the network of factors controlling mitochondrial functions, i. e., morphogenesis machinery and metabolic sensor proteins, produce metabolic inflexibility, insulin resistance and reduced oxidative capacity in skeletal muscle. Moreover, intramyocellular lipid (IMCL) accumulation leads to tissue damage and inflammation. The administration of the naturally occurring metabolite 3,5-diiodo-L-thyronine (T2) with thyromimetic actions to high fat diet (HFD)-fed rats exerts a systemic hypolipidemic effect, which produces a lack of IMCL accumulation, a shift toward glycolytic fibers and amelioration of insulin sensitivity in gastrocnemius muscle. In this study, an integrated approach combining large-scale expression profile and functional analyses was used to characterize the response of skeletal muscle mitochondria to T2 during a HFD regimen. Long-term T2 administration to HDF rats induced a glycolytic phenotype of gastrocnemius muscle as well as an adaptation of mitochondria to the fiber type, with a decreased representation of enzymes involved in mitochondrial oxidative metabolism. At the same time, T2 stimulated the activity of individual respiratory complex I, IV, and V. Moreover, T2 prevented the HFD-associated increase in the expression of peroxisome proliferative activated receptor γ coactivator-17alpha; and dynamin-1-like protein as well as mitochondrial morphological aberrations, favoring the appearance of tubular and tethered organelles in the intermyofibrillar regions. Remarkably, T2 reverted the HDF-associated expression pattern of proinflammatory factors, such as p65 subunit of NF-kB, and increased the fiber-specific immunoreactivity of adipose differentiation-related protein in lipid droplets. All together, these results further support a role of T2 in counteracting in vivo some of the HFD-induced impairment in structural/metabolic features of skeletal muscle by impacting the mitochondrial phenotype.",
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AU - De Matteis, Rita

AU - Senese, Rosalba

AU - de Lange, Pieter

AU - Coppola, Maria

AU - Salzano, Anna M.

AU - Scaloni, Andrea

AU - Ceccarelli, Michele

AU - Goglia, Fernando

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