[12] Initiation of Globin Synthesis: Assays

Ronald Crystal, Norton A. Elson, W. French Anderson

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Several additional assays have been developed in the laboratory for the study of the globin initiation process, but the assays described here have been most useful. The division into two major groups (those using artificial templates and those using natural mRNA templates) has been made because the former are easier to use (and are useful therefore for purification of the initiation factors), while the latter require more care but are more valuable in understanding the mechanism of the initiation process. Although each assay has many uses, it has been found that certain assays are more valuable for a specific purpose or for understanding a specific mechanism (e.g., GTP requirements in initiator tRNA binding).

Original languageEnglish
Pages (from-to)101-128
Number of pages28
JournalMethods in Enzymology
Volume30
Issue numberC
DOIs
Publication statusPublished - 1 Jan 1974
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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