α-Synuclein Levels in Blood Plasma from LRRK2 Mutation Carriers

Ana Gorostidi, Alberto Bergareche, Javier Ruiz-Martínez, José F. Martí-Massó, María Cruz, Shiji Varghese, Mohamed M. Qureshi, Fatimah Alzahmi, Abdulmonem Al-Hayani, Adolfo López de Munáin, Omar M.A. El-Agnaf

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26 Citations (Scopus)

Abstract

The diagnosis of Parkinson's disease (PD) remains primarily a clinical issue, based mainly on phenotypic patterns. The identification of biomarkers capable of permitting the preclinical detection of PD is critically needed. α-Synuclein is a key protein in PD, with missense and multiplication mutations in the gene encoding α-synuclein (SNCA) having been reported in familial cases of PD, and accumulation of the protein identified in Lewy bodies (LBs) and Lewy neurites (LNs) in affected brain regions. With the objective of validating the use of α-synuclein as a clinical or progressive biomarker in an accessible tissue, we used an enzyme-linked immunosorbent assay (ELISA) to measure α-synuclein levels in the peripheral blood plasma of idiopathic PD and LRRK2 mutation carrier patients and compared our findings with healthy control subjects. Compared to healthy controls, we found a significant decrease in plasma total α-synuclein levels in idiopathic PD (iPD) patients (n = 134, p = 0.010). However, the reduction was less significant in patients who were LRRK2 mutation carriers (n = 32, p = 0.133). This lack of significance could be due to the small number of individuals employed in this group. No predictive value of total α-synuclein in the diagnosis of PD was found in a receiver operating characteristic (ROC) curve analysis. Although this is a pilot study requiring corroboration on a larger cohort of patients, our results highlight the possible use of plasma α-synuclein as a biomarker for PD.

Original languageEnglish
Article numbere52312
JournalPloS one
Volume7
Issue number12
DOIs
Publication statusPublished - 27 Dec 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Gorostidi, A., Bergareche, A., Ruiz-Martínez, J., Martí-Massó, J. F., Cruz, M., Varghese, S., Qureshi, M. M., Alzahmi, F., Al-Hayani, A., de Munáin, A. L., & El-Agnaf, O. M. A. (2012). α-Synuclein Levels in Blood Plasma from LRRK2 Mutation Carriers. PloS one, 7(12), [e52312]. https://doi.org/10.1371/journal.pone.0052312